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Manco Inc e. coli bl21 (de3)- sacpox
Shake flask growth curves of <t>E.</t> <t>coli-Sacpox</t> ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]
E. Coli Bl21 (De3) Sacpox, supplied by Manco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+bl21+(de3)-+sacpox/escherichia+coli+strain+bl21+de3+/pmc05861644-60-1-20
Average 90 stars, based on 1 article reviews
e. coli bl21 (de3)- sacpox - by Bioz Stars, 2026-10
90/100 stars

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Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers

Journal: BMC Biotechnology

doi: 10.1186/s12896-018-0427-0

Shake flask growth curves of E. coli-Sacpox ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]
Figure Legend Snippet: Shake flask growth curves of E. coli-Sacpox ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]

Techniques Used: Comparison

Batch experiments (2.5 L) of E. coli-Sacpox and E. coli-Ssopox 3 M induced with 1.0 mM IPTG, 5.0 or 10.0 mM galactose at around 6.0 Abs 600nm , as indicated by the arrows: growth curves, IPTG or galactose up-take ( a - b ); Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the different batch experiments ( c - d )
Figure Legend Snippet: Batch experiments (2.5 L) of E. coli-Sacpox and E. coli-Ssopox 3 M induced with 1.0 mM IPTG, 5.0 or 10.0 mM galactose at around 6.0 Abs 600nm , as indicated by the arrows: growth curves, IPTG or galactose up-take ( a - b ); Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the different batch experiments ( c - d )

Techniques Used:

Fed-batch experiments in 2.5 and 22.0-L vessels of E. coli Sacpox and E. coli Ssopox 3 M induced with 10.0 mM galactose at about 40.0 Abs 600nm , as indicated by the arrows: growth curves, galactose up-take, glycerol consumption, acetic acid formation and feeding profile ( a - b ). Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the 2.5 and 22.0-L fed-batch experiments ( c - d )
Figure Legend Snippet: Fed-batch experiments in 2.5 and 22.0-L vessels of E. coli Sacpox and E. coli Ssopox 3 M induced with 10.0 mM galactose at about 40.0 Abs 600nm , as indicated by the arrows: growth curves, galactose up-take, glycerol consumption, acetic acid formation and feeding profile ( a - b ). Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the 2.5 and 22.0-L fed-batch experiments ( c - d )

Techniques Used:

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Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers
Article Snippet: The strains E. coli BL21 (DE3)- Sacpox and E. coli BL21 (DE3)- Ssopox 3 M (C258L/I261F/W263A) were obtained by Prof. Manco by employing genetic engineering strategies, as previously described [ , ], and they were stored at − 80 °C in 20% ( v /v) glycerol stock solutions.



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Manco Inc e. coli bl21 (de3)- sacpox
Shake flask growth curves of <t>E.</t> <t>coli-Sacpox</t> ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]
E. Coli Bl21 (De3) Sacpox, supplied by Manco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+bl21+(de3)-+sacpox/escherichia+coli+strain+bl21+de3+/pmc05861644-60-1-20
Average 90 stars, based on 1 article reviews
e. coli bl21 (de3)- sacpox - by Bioz Stars, 2026-10
90/100 stars
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Shake flask growth curves of E. coli-Sacpox ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]

Journal: BMC Biotechnology

Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers

doi: 10.1186/s12896-018-0427-0

Figure Lengend Snippet: Shake flask growth curves of E. coli-Sacpox ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]

Article Snippet: The strains E. coli BL21 (DE3)- Sacpox and E. coli BL21 (DE3)- Ssopox 3 M (C258L/I261F/W263A) were obtained by Prof. Manco by employing genetic engineering strategies, as previously described [ , ], and they were stored at − 80 °C in 20% ( v /v) glycerol stock solutions.

Techniques: Comparison

Batch experiments (2.5 L) of E. coli-Sacpox and E. coli-Ssopox 3 M induced with 1.0 mM IPTG, 5.0 or 10.0 mM galactose at around 6.0 Abs 600nm , as indicated by the arrows: growth curves, IPTG or galactose up-take ( a - b ); Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the different batch experiments ( c - d )

Journal: BMC Biotechnology

Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers

doi: 10.1186/s12896-018-0427-0

Figure Lengend Snippet: Batch experiments (2.5 L) of E. coli-Sacpox and E. coli-Ssopox 3 M induced with 1.0 mM IPTG, 5.0 or 10.0 mM galactose at around 6.0 Abs 600nm , as indicated by the arrows: growth curves, IPTG or galactose up-take ( a - b ); Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the different batch experiments ( c - d )

Article Snippet: The strains E. coli BL21 (DE3)- Sacpox and E. coli BL21 (DE3)- Ssopox 3 M (C258L/I261F/W263A) were obtained by Prof. Manco by employing genetic engineering strategies, as previously described [ , ], and they were stored at − 80 °C in 20% ( v /v) glycerol stock solutions.

Techniques:

Fed-batch experiments in 2.5 and 22.0-L vessels of E. coli Sacpox and E. coli Ssopox 3 M induced with 10.0 mM galactose at about 40.0 Abs 600nm , as indicated by the arrows: growth curves, galactose up-take, glycerol consumption, acetic acid formation and feeding profile ( a - b ). Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the 2.5 and 22.0-L fed-batch experiments ( c - d )

Journal: BMC Biotechnology

Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers

doi: 10.1186/s12896-018-0427-0

Figure Lengend Snippet: Fed-batch experiments in 2.5 and 22.0-L vessels of E. coli Sacpox and E. coli Ssopox 3 M induced with 10.0 mM galactose at about 40.0 Abs 600nm , as indicated by the arrows: growth curves, galactose up-take, glycerol consumption, acetic acid formation and feeding profile ( a - b ). Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the 2.5 and 22.0-L fed-batch experiments ( c - d )

Article Snippet: The strains E. coli BL21 (DE3)- Sacpox and E. coli BL21 (DE3)- Ssopox 3 M (C258L/I261F/W263A) were obtained by Prof. Manco by employing genetic engineering strategies, as previously described [ , ], and they were stored at − 80 °C in 20% ( v /v) glycerol stock solutions.

Techniques: