e. coli bl21 (de3)- sacpox (Manco Inc)
Structured Review
![Shake flask growth curves of <t>E.</t> <t>coli-Sacpox</t> ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1644/pmc05861644/pmc05861644__12896_2018_427_Fig2_HTML.jpg)
E. Coli Bl21 (De3) Sacpox, supplied by Manco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers"
Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers
Journal: BMC Biotechnology
doi: 10.1186/s12896-018-0427-0
Figure Legend Snippet: Shake flask growth curves of E. coli-Sacpox ( a ) and E. coli-Ssopox 3 M ( b ) induced with 0.01, 0.1, 0.5, 1.0 mM IPTG, 5.0 mM or 10.0 mM galactose at about 1.0 Abs 600nm , as indicated by the arrows, compared with a not-induced growth; comparison of Sac Pox and Sso Pox 3 M enzyme production (U·g cww −1 ) in the different shake flask experiments ( c ). [*p < 0.05 compared to the not- induced shake flask; **p < 0.05 compared to the IPTG induced shake flask]
Techniques Used: Comparison
Figure Legend Snippet: Batch experiments (2.5 L) of E. coli-Sacpox and E. coli-Ssopox 3 M induced with 1.0 mM IPTG, 5.0 or 10.0 mM galactose at around 6.0 Abs 600nm , as indicated by the arrows: growth curves, IPTG or galactose up-take ( a - b ); Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the different batch experiments ( c - d )
Techniques Used:
Figure Legend Snippet: Fed-batch experiments in 2.5 and 22.0-L vessels of E. coli Sacpox and E. coli Ssopox 3 M induced with 10.0 mM galactose at about 40.0 Abs 600nm , as indicated by the arrows: growth curves, galactose up-take, glycerol consumption, acetic acid formation and feeding profile ( a - b ). Sac Pox and Sso Pox 3 M enzyme production (U·L − 1 ) in the 2.5 and 22.0-L fed-batch experiments ( c - d )
Techniques Used:
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Comparison:Article Title: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers Article Snippet: The strains E. coli BL21 (DE3)- Sacpox and E. coli BL21 (DE3)- Ssopox 3 M (C258L/I261F/W263A) were obtained by |